For Research Use Only
DSIP is supplied exclusively for in vitro laboratory research. Not for human or veterinary use. No dosing, reconstitution, or administration guidance is provided.
DSIP 10mg — Delta Sleep-Inducing Peptide: A High-Uncertainty Research Nonapeptide
DSIP 10mg is a research-grade lyophilised preparation of delta sleep-inducing peptide (DSIP), a synthetic nonapeptide with the sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu. Each vial supplied by AbsoluteBioLab contains 10mg of DSIP, verified by RP-HPLC to ≥99.0% purity with identity confirmed by ESI-MS. Batch-specific analytical documentation is available through the AbsoluteBioLab CoA Portal.
Evidence uncertainty notice: DSIP is a high-uncertainty research compound. The original sleep-factor hypothesis — that DSIP is an endogenous sleep-inducing substance — is poorly documented and remains unresolved in the scientific literature. A major review of DSIP research has explicitly described the sleep-factor hypothesis as inconsistent with the available evidence. Researchers should approach DSIP with appropriate scepticism regarding its proposed endogenous functions and should not treat the name “delta sleep-inducing peptide” as proof of a reliable or reproducible sleep effect. The compound is supplied here for laboratory investigation of its biochemical properties, not as a sleep aid or sleep-regulating agent.
Compound Identity
| Attribute | Value | Notes |
|---|---|---|
| Common Name | DSIP, Delta Sleep-Inducing Peptide | Name reflects original experimental context; sleep-factor hypothesis remains unresolved |
| Sequence | Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu | 9 amino acids; free N-terminus, free C-terminus (verify terminal modifications against batch CoA) |
| CAS Number | 62568-57-4 | DSIP (free acid); verify salt form against batch CoA |
| Molecular Formula | C35H48N10O15 | Free acid |
| Molecular Weight | 848.8 Da | Free acid (average) |
| Salt Form | Acetate | Research-grade lyophilised preparation; verify against batch CoA |
| Physical Form | White lyophilised powder | Research-grade preparation |
Analytical Specification
| Test Parameter | Method | Specification |
|---|---|---|
| Purity (RP-HPLC) | C18 column, UV 220 nm | ≥99.0% |
| Identity (ESI-MS) | Electrospray ionisation MS | ±0.1 Da of theoretical MW (849.8 Da [M+H]⁺) |
| Endotoxin (LAL) | Kinetic turbidimetric LAL | <1.0 EU/mg |
| Appearance | Visual inspection | White lyophilised powder |
Evidence Context and Literature Uncertainty
DSIP was originally isolated from rabbit cerebral venous blood by Monnier and colleagues in 1977 and described as a factor capable of inducing slow-wave (delta) sleep in rabbits when infused into recipient animals. Subsequent research attempted to characterise DSIP as an endogenous sleep-regulating substance, but the results were inconsistent and often non-reproducible across laboratories and species.
A comprehensive review of DSIP research (Graf and Kastin, 1984, and subsequent reviews) explicitly described the sleep-factor hypothesis as poorly documented and noted that the available evidence did not support DSIP as a reliable endogenous sleep-inducing substance. Key issues identified in the literature include: (1) inconsistent sleep effects across species and experimental conditions; (2) difficulty in reproducing the original sleep-induction findings; (3) uncertainty about whether DSIP crosses the blood-brain barrier in physiologically relevant quantities; (4) absence of a well-characterised specific receptor for DSIP; (5) inconsistent findings regarding endogenous DSIP concentrations and their relationship to sleep states.
DSIP has also been studied in the context of stress regulation, cortisol modulation, and nociception in animal models, but these findings are similarly preliminary and should not be presented as established mechanisms. Researchers using DSIP should treat it as a compound with a historically interesting but scientifically unresolved profile, and should design experiments with appropriate controls for non-specific effects.
Research Applications
Neuropeptide Biology Research
DSIP is used as a tool compound in research investigating neuropeptide biology, including the biochemical properties of small neuropeptides and their interactions with neural tissue in cell-based and animal models. The compound’s well-characterised sequence and commercial availability make it a useful reference compound for neuropeptide research, independent of the unresolved sleep-factor hypothesis.
Historical Sleep Research Context
DSIP has a substantial historical literature in sleep research, making it relevant for researchers studying the history of sleep-factor research or investigating the biochemical basis of sleep regulation in animal models. Researchers should approach this literature with awareness of the evidence limitations described above and should not design experiments based solely on the assumption that DSIP reliably induces delta sleep in the experimental system being used.
Storage and Transport
| Condition | Specification |
|---|---|
| Long-Term Storage | −20°C, protected from light and moisture |
| Stability Period | Stated on the batch-specific CoA |
| Transit | Ambient; cold-chain packaging included for applicable orders |
Batch Documentation
Every batch of DSIP 10mg dispatched by AbsoluteBioLab is accompanied by a batch-specific Certificate of Analysis. CoA documents are available via the Certificate of Analysis Portal. For further information on research compound quality and documentation, refer to the Supplier Verification and Quality System.
Frequently Asked Questions
Does DSIP reliably induce sleep?
The sleep-inducing activity of DSIP is not reliably established. The original sleep-factor hypothesis, based on experiments in rabbits in the 1970s, has not been consistently reproduced across species and experimental conditions. A major review of DSIP research described the sleep-factor hypothesis as poorly documented. Researchers should not assume that DSIP will reliably induce sleep in their experimental system and should design experiments with appropriate controls.
What receptor does DSIP bind to?
A specific, well-characterised receptor for DSIP has not been identified in the published literature. DSIP’s mechanism of action, if any, remains unclear. Researchers should not assume a specific receptor target without independent experimental evidence in their specific model system.
Where can I access the Certificate of Analysis?
Batch-specific CoA documents are available via the AbsoluteBioLab CoA Portal.
Reviews
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